Comparisons of virulence of influenza virus recombinants in ferrets in relation to their behaviour in man and their genetic constitution.
Identifieur interne : 002880 ( Main/Exploration ); précédent : 002879; suivant : 002881Comparisons of virulence of influenza virus recombinants in ferrets in relation to their behaviour in man and their genetic constitution.
Auteurs : D. Campbell ; C. Sweet ; H. SmithSource :
- The Journal of general virology [ 0022-1317 ] ; 1979.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Virus de la grippe A.
- isolement et purification : Virus de la grippe A.
- microbiologie : Grippe humaine.
- métabolisme : Muqueuse nasale.
- pathogénicité : Virus de la grippe A.
- Animaux, Furets, Gènes viraux, Humains, Recombinaison génétique, Virulence.
English descriptors
- KwdEn :
- MESH :
- genetics : Influenza A virus.
- isolation & purification : Influenza A virus.
- metabolism : Nasal Mucosa.
- microbiology : Influenza, Human.
- pathogenicity : Influenza A virus.
- Animals, Ferrets, Genes, Viral, Humans, Recombination, Genetic, Virulence.
Abstract
Two parent viruses, A/Finland/4/74(H3N2) and A/Okuda/57(H2N2), virulent and attenuated respectively for man, showed similar differences of virulence in ferrets as judged by estimations of 50% minimal infectious doses (MID50), the level and persistence of nasal infection, the height and duration of pyrexia and the level of lung infection. In ferrets, two recombinant clones, WRL 94(H3N2) and WRL 105(H3N2), were almost as virulent as A/Finland and indistinguishable from one another, a result which agreed well with genetic analysis (Hay et al. 1977); the RNA pieces of these recombinants appeared identical and largely derived from the virulent parent (A/Finland). The results in ferrets did not agree with tests on clone WRL 94 in small numbers of human volunteers but they were not inconsistent with those on clone WRL 105 in larger numbers. It is possible therefore that careful tests in ferrets may yield more accurate information on the virulence of strains than limited tests in human volunteers. A rapid test for virulence in ferrets is described. It could be used to screen many additional recombinants thereby yielding information on the genetical basis of virulence and indicating possible vaccine strains for more thorough testing in ferrets and in man.
DOI: 10.1099/0022-1317-44-1-37
PubMed: 501338
Affiliations:
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Le document en format XML
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Smith</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1979">1979</date><idno type="RBID">pubmed:501338</idno><idno type="pmid">501338</idno><idno type="doi">10.1099/0022-1317-44-1-37</idno><idno type="wicri:Area/PubMed/Corpus">000606</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000606</idno><idno type="wicri:Area/PubMed/Curation">000606</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000606</idno><idno type="wicri:Area/PubMed/Checkpoint">000528</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000528</idno><idno type="wicri:Area/Ncbi/Merge">001137</idno><idno type="wicri:Area/Ncbi/Curation">001137</idno><idno type="wicri:Area/Ncbi/Checkpoint">001137</idno><idno type="wicri:doubleKey">0022-1317:1979:Campbell D:comparisons:of:virulence</idno><idno type="wicri:Area/Main/Merge">002A30</idno><idno type="wicri:Area/Main/Curation">002880</idno><idno type="wicri:Area/Main/Exploration">002880</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparisons of virulence of influenza virus recombinants in ferrets in relation to their behaviour in man and their genetic constitution.</title><author><name sortKey="Campbell, D" sort="Campbell, D" uniqKey="Campbell D" first="D" last="Campbell">D. Campbell</name></author><author><name sortKey="Sweet, C" sort="Sweet, C" uniqKey="Sweet C" first="C" last="Sweet">C. Sweet</name></author><author><name sortKey="Smith, H" sort="Smith, H" uniqKey="Smith H" first="H" last="Smith">H. Smith</name></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="1979" type="published">1979</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Ferrets</term><term>Genes, Viral</term><term>Humans</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (isolation & purification)</term><term>Influenza A virus (pathogenicity)</term><term>Influenza, Human (microbiology)</term><term>Nasal Mucosa (metabolism)</term><term>Recombination, Genetic</term><term>Virulence</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Furets</term><term>Grippe humaine (microbiologie)</term><term>Gènes viraux</term><term>Humains</term><term>Muqueuse nasale (métabolisme)</term><term>Recombinaison génétique</term><term>Virulence</term><term>Virus de la grippe A (génétique)</term><term>Virus de la grippe A (isolement et purification)</term><term>Virus de la grippe A (pathogénicité)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Nasal Mucosa</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Muqueuse nasale</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Ferrets</term><term>Genes, Viral</term><term>Humans</term><term>Recombination, Genetic</term><term>Virulence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Furets</term><term>Gènes viraux</term><term>Humains</term><term>Recombinaison génétique</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Two parent viruses, A/Finland/4/74(H3N2) and A/Okuda/57(H2N2), virulent and attenuated respectively for man, showed similar differences of virulence in ferrets as judged by estimations of 50% minimal infectious doses (MID50), the level and persistence of nasal infection, the height and duration of pyrexia and the level of lung infection. In ferrets, two recombinant clones, WRL 94(H3N2) and WRL 105(H3N2), were almost as virulent as A/Finland and indistinguishable from one another, a result which agreed well with genetic analysis (Hay et al. 1977); the RNA pieces of these recombinants appeared identical and largely derived from the virulent parent (A/Finland). The results in ferrets did not agree with tests on clone WRL 94 in small numbers of human volunteers but they were not inconsistent with those on clone WRL 105 in larger numbers. It is possible therefore that careful tests in ferrets may yield more accurate information on the virulence of strains than limited tests in human volunteers. A rapid test for virulence in ferrets is described. It could be used to screen many additional recombinants thereby yielding information on the genetical basis of virulence and indicating possible vaccine strains for more thorough testing in ferrets and in man.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Campbell, D" sort="Campbell, D" uniqKey="Campbell D" first="D" last="Campbell">D. Campbell</name><name sortKey="Smith, H" sort="Smith, H" uniqKey="Smith H" first="H" last="Smith">H. Smith</name><name sortKey="Sweet, C" sort="Sweet, C" uniqKey="Sweet C" first="C" last="Sweet">C. Sweet</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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